Isolation of Temperature-Sensitive Conditional Lethal Mutants of “Fixed” Rabies Virus
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چکیده
منابع مشابه
Isolation of temperature-sensitive conditional lethal mutants of "fixed" rabies virus.
In an attempt to induce temperature-sensitive (ts) conditional lethal mutants of rabies virus, stocks of a plaque-purified substrain of strain CVS fixed rabies virus were subjected to mutagenesis by HNO(2), 5-fluorouracil, or 5-azacytidine. It was necessary to prepare virus stocks from clones of mutagenized virus selected at random and to test subsequently each stock for possible ts characteris...
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Ten temperature-sensitive mutants of Sendai virus, a paramyxovirus, were isolated and partially characterized. The mutants replicated in chicken embryo lung cells at 30 C, but not at 38 C; wild-type virus grew equally well at both temperatures. Complementation tests divided the mutants into seven groups. Six groups synthesized neither infectious virus nor RNA when incubated at 38 C from the beg...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1971
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.7.3.295-300.1971